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expression plasmid pcmv3 msnx10 ha  (Sino Biological)


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    Structured Review

    Sino Biological expression plasmid pcmv3 msnx10 ha
    Expression Plasmid Pcmv3 Msnx10 Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pcmv3/pCMV3-C-HA+Negative+Control+Vector/pm42043249-86-3-13
    Average 94 stars, based on 51 article reviews
    expression plasmid pcmv3 msnx10 ha - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling
    Article Snippet: Small interfering RNAs (siRNAs) against human PBX3, USP14, and USF1 were purchased from RiboBio Co., Ltd (Guangzhou, China), and non-specific siRNA was used as a negative control. .. Flag-ATRAP plasmid (Flag-ATRAP), PBX3 plasmid (PBX3), and USF1 plasmid (USF1) were subcloned into the expression vector pCMV3 (Sino Biological Inc., Beijing, China), while empty vector plasmid was used as a negative control. .. All of the siRNAs and plasmids were transfected using jetPRIME ® (Polyplus-transfection S.A, Illkirch, France) according to the manufacturer's instructions.

    Article Title: HSP90 inhibition suppresses tumor glycolytic flux to potentiate the therapeutic efficacy of radiotherapy for head and neck cancer
    Article Snippet: The full-length HA-tagged human PKM gene was cloned into the pDONR-221 lentiviral expression vector (Invitrogen) as we previously described ( ). pcDNA3.1-PFKP containing human PFKP gene was provided by Z. Lu ( ). .. The full-length Flag-tagged human IL8 was cloned into mammalian expression vector pCMV3 and obtained from SinoBiological Inc. (catalog no. HG10098-CF). .. Lentiviral plasmids, together with packaging plasmids were cotransfected into Lenti-Pac 293TA cells (GeneCopoeia, Rockville, MD) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.

    Expressing:

    Article Title: USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling
    Article Snippet: Small interfering RNAs (siRNAs) against human PBX3, USP14, and USF1 were purchased from RiboBio Co., Ltd (Guangzhou, China), and non-specific siRNA was used as a negative control. .. Flag-ATRAP plasmid (Flag-ATRAP), PBX3 plasmid (PBX3), and USF1 plasmid (USF1) were subcloned into the expression vector pCMV3 (Sino Biological Inc., Beijing, China), while empty vector plasmid was used as a negative control. .. All of the siRNAs and plasmids were transfected using jetPRIME ® (Polyplus-transfection S.A, Illkirch, France) according to the manufacturer's instructions.

    Article Title: HSP90 inhibition suppresses tumor glycolytic flux to potentiate the therapeutic efficacy of radiotherapy for head and neck cancer
    Article Snippet: The full-length HA-tagged human PKM gene was cloned into the pDONR-221 lentiviral expression vector (Invitrogen) as we previously described ( ). pcDNA3.1-PFKP containing human PFKP gene was provided by Z. Lu ( ). .. The full-length Flag-tagged human IL8 was cloned into mammalian expression vector pCMV3 and obtained from SinoBiological Inc. (catalog no. HG10098-CF). .. Lentiviral plasmids, together with packaging plasmids were cotransfected into Lenti-Pac 293TA cells (GeneCopoeia, Rockville, MD) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.

    Negative Control:

    Article Title: USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling
    Article Snippet: Small interfering RNAs (siRNAs) against human PBX3, USP14, and USF1 were purchased from RiboBio Co., Ltd (Guangzhou, China), and non-specific siRNA was used as a negative control. .. Flag-ATRAP plasmid (Flag-ATRAP), PBX3 plasmid (PBX3), and USF1 plasmid (USF1) were subcloned into the expression vector pCMV3 (Sino Biological Inc., Beijing, China), while empty vector plasmid was used as a negative control. .. All of the siRNAs and plasmids were transfected using jetPRIME ® (Polyplus-transfection S.A, Illkirch, France) according to the manufacturer's instructions.

    Clone Assay:

    Article Title: HSP90 inhibition suppresses tumor glycolytic flux to potentiate the therapeutic efficacy of radiotherapy for head and neck cancer
    Article Snippet: The full-length HA-tagged human PKM gene was cloned into the pDONR-221 lentiviral expression vector (Invitrogen) as we previously described ( ). pcDNA3.1-PFKP containing human PFKP gene was provided by Z. Lu ( ). .. The full-length Flag-tagged human IL8 was cloned into mammalian expression vector pCMV3 and obtained from SinoBiological Inc. (catalog no. HG10098-CF). .. Lentiviral plasmids, together with packaging plasmids were cotransfected into Lenti-Pac 293TA cells (GeneCopoeia, Rockville, MD) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.



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